Cryo-EM tutorial days (2016)

ECTS credits:

1 ECTS

 Course parameters:

Language: English

Level of course: PhD course

Time of year: Q2 2016

No. of contact hours/hours in total incl. preparation, assignment(s) or the like: 15/30 hours

Capacity limits: 40 participants

Objectives of the course:
Single-particle cryo-electron microscopy (cryo-EM) is a structure determination technique that is currently gaining popularity in structural biology. Through the availability of powerful computers, novel transmission electron microscopes and especially modern electron detectors the method now reach near atomic resolution that competes with traditional methods of X-ray crystallography and nuclear magnetic resonance. Cryo-EM further allows the study of sample heterogeneity and dynamics, and cryo-electron tomography (ET) allows single particles to be observed in their native environment inside cells. This course aims at providing an overview of current cryo-EM research and method development as well as giving participants practical experience with software tools for cryo-EM data analysis.

 Learning outcomes and competences:
At the end of the course, the student should be able to:

  • Account for central research topics and methodology of cryo-EM
  • Compare and choose between different sample preparation methods for cryo-EM
  • Identify and apply appropriate software analysis tools for cryo-EM data
  • Analyze and construct 3D models based on cryo-EM data
  • Apply alternative methods for analyzing heterogeneous samples
  • Describe and critically evaluate 3D reconstructions based on cryo-EM data

 Compulsory programme:
Active participation and tutorial exercises

 Course contents:

  • Introduction to the Aarhus cryo-EM facility
  • Sample preparation methods for cryo-EM
  • Cryo-electron tomography
  • Single-particle analysis using RELION
  • Biomolecular nanotechnology as novel tool for cryo-EM
  • Dynamics of membrane transporters
  • Helical reconstruction using SPRING

 Prerequisites:
None

 Organizers:

 Name of lecturer[s]:

 Type of course/teaching methods:
Lectures and exercises

 Literature:
Reviews, research papers and tutorial material

 Course homepage:
http://inano.au.dk/cryo-em-days

 Course assessment:
None

Provider:
Interdisciplinary Nanoscience Center (iNANO)

 Special comments on this course:
The course also serves as the inauguration of the first Danish cryo-EM facility including the flagship Titan Krios microscope with post-Quantum Gatan K2 detector.

 Time:

  • Thursday, 1 December, 9.00-19.00
  • Friday, 2 December, 9.00-17.00

 Place:
iNANO Auditorium (1593-012), Gustav Wieds Vej 14, 8000 Aarhus C

 Registration:
Deadline for registration is Wednesday, November 23, 2016. Information regarding admission will be sent out no later than Friday, November 23, 2016.

For registration: http://webforms.au.dk?form=1573

If you have any questions, please contact Ebbe Sloth Andersen, e-mail: esa@inano.au.dk

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