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Introduction to metabolomics 2018

Name of course:

Introduction to metabolomics

 

ECTS credits:

2

 

Course parameters:

Language: English

Level of course: PhD course (with participation of a few master students)

Time of year: January-February 2018

No. of contact hours/hours in total incl. preparation, assignment(s) or the like:

3 days of lectures and exercises (24 h)

Preparation by reading book chapters, articles and preparing data for analysis (20 h)

Writing report (16 h)

Capacity limits: max 25, min 10. PhD students have highest priority

 

Objectives of the course:

The purpose of the course is to give an up-to-date introduction to the basic methods in metabolomics. The course will cover nuclear magnetic resonance (NMR) spectroscopy and gas and liquid chromatography mass spectrometry (GC/LC-MS) methodologies used in metabolomics, as well as design of experiments.

 

Learning outcomes and competences:

At the end of the course, the student should be able to:

·          Arrange the place of metabolomics in context of genomics, proteomics, *omics

·          Compare and contrast the methods for a metabolomics study considering the benefits and the pitfalls of the methods

·          Describe and explain how to prepare samples from different foods and bio-fluids for metabolomic analyses

·          Evaluate and apply the appropriate experimental design in a metabolomics study

·          Describe appropriate design of experiments for metabolomic analyses

·          Explain the principles of metabolite detection and identification in LC-MS, GC-MS and NMR

·          Outline applications of metabolomics to your field of research

·          Apply multivariate data analysis methods relevant for metabolomic data

·          Critically evaluate metabolomic data described in scientific literature and describe your own results in a scientific way


Compulsory programme:

Attendance for a minimum of 80% of the theoretical and practical lessons is required to obtain the course diploma. Approved report.

 

Course contents:

Metabolism is the set of chemical reactions that occur in living organisms in order to maintain life and metabolites is the end products of these chemical reactions. Metabolomics is the systematic study of metabolite profiles in any biological sample and tissue. Set in a systems biology context, the metabolomics is the most recent member of the omics-family and it is complementary to genomics and proteomics.

The course gives an introduction to the use of metabolomics and its methods. The objectives are to give participants knowledge of advanced NMR and MS techniques, including hands-on experience with processing, analysis and identification of metabolites in such data.

Theoretical and practical aspects of these methods, including design of experiments, sample preparation, data acquisition, processing and further analysis will be discussed. The course will cover NMR- and MS-based metabolomics applied to various types of samples – meat and vegetable extracts, cells, milk, urine, blood, and feces – will be used as examples.

 

Prerequisites:

Enrolled in a science based PhD programme. Master students can participate as a part of their master project in agreement with the supervisor.

 

Name of lecturers:

Postdoc Ulrik Kræmer Sundekilde, Department of Food Science, Aarhus University, Denmark

PhD student Louise MA Jakobsen, Department of Food Science, Aarhus University, Denmark

PhD student Rebekka Thøgersen, Department of Food Science, Aarhus University, Denmark

 

Type of course/teaching methods:

Lectures and exercises

 

Literature:

Selected chapters and papers will be announced on BlackBoard. During the course PLS Toolbox or Solo (Eigenvector) will be used for data analysis. You need to install the software at your computer. You will receive a folder with information and printouts from the lectures at the beginning of the course.

 

Course homepage:

None

 

Course assessment:

Passed/not passed assessment based on the written report considering the learning outcomes.

 

Provider:

Department of Food Science

 

Special comments on this course:

The course is organized in combination with the PhD course ‘Introduction to multivariate data analysis’ and it is encouraged to follow both courses, although the courses can be taken individually if necessary. There will be a discount if you follow both courses. The fee also covers coffee and bread in the morning and afternoon.

 

Time:

Metabolomics only:

Thursday 18 January: Lectures

Friday 19 January: Lectures

Wednesday 24 January: Data preparation, workshop, lectures

 

Thursday-Friday 25 January-2 February: Report work

Friday 2 February at 24:00: Deadline for handing in report

 

Friday 9 February: Notice of assessment

 

Chemometrics only:

Monday 22 January: Lectures

Tuesday 23 January: Lectures

Wednesday 24 January: Study home day

Thursday 25 January: Lectures

Friday 26 January: Working with own data

 

Monday-Thursday 29 January – 2 February: Report work

 

Friday 2 February at 24:00: Deadline for handing in report

Thursday 8 February: Examination seminar (peer-feedback, teacher-feedback)

 

Metabolomics and Chemometrics:

Thursday 18 January: Lectures

Friday 19 January: Lectures

 

Monday 22 January: Lectures

Tuesday 23 January: Lectures

Wednesday 24 January: Data preparation, workshop lectures (Metabolomics only; chemometrics study home)

Thursday 25 January: Lectures

Friday 26 January: Working with own data

 

Thursday-Friday 25 January-2 February: Report work

Friday 2 February at 24:00: Deadline for handing in report

 

Thursday 8 February: Examination seminar (peer-feedback, teacher-feedback)

Friday 9 February: Notice of assessment (metabolomics only)

 

Place:

Aarhus

 

Registration:

Deadline for registration is 20 December 2017. Information regarding admission will be sent out no later than two workdays after registration.

 

For registration: https://auws.au.dk/Metabolomics2017

 

If you have any questions, please contact Ulrik Sundekilde, e-mail: uksundekilde@food.au.dk

 

The course fee is:

Metabolomics only:

PhD students and master students enrolled at Danish Universities and AU-FOOD staff: 750 DKK

Others: 3000 DKK

 

Chemometrics only:

PhD students and master students enrolled at Danish Universities and AU-FOOD staff: 1750 DKK

Others: 5000 DKK

 

Metabolomics and Chemometrics

PhD students and master students enrolled at Danish Universities and AU-FOOD staff: 2000 DKK

Others: 7500 DKK

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Revised 03.08.2017