Development of a metabolomics platform to elucidate the effects of polydextose intake on activity of the gut microbiota

PhD defence, Tuesday 25 April 2017. Santosh Lamichhane.

2017.04.25 | Steffi Hjerrild Iversen

Santosh Lamichhane

The objective of Santosh Lamichhane’s PhD project was to develop and employ NMR-based metabolomics as a tool to characterize the impact of polydextrose (PDX), a synthetic soluble fiber on the activity of the gut microbiota. Recently, fecal metabolomics is gaining increasing attention for probing gut microbial activity, which is believed to play a vital role in the regulation of human metabolism and disease prevention.  

During his research, the assessment of metabolic changes in the human and in-vitro colon by using metabolomics enabled identification of specific human intestinal metabolites generated from PDX fiber fermentation. These metabolites are expected to play a vital role in the regulation of host metabolism and disease prevention.  Santosh Lamichhane implemented an advanced multilevel data analysis approach in his study, which enabled the identification of the specific inter- and intra-individual variations and fiber-induced effects on the fecal metabolome. His thesis also presents considerations around sample preparation and analytical strategies for NMR-based metabolomics of human feces.

Overall, the research has contributed to the development of novel strategies for a metabolomics platform that enable insight into fiber fermentation, gut-microbes interactions and that aid to further understand how the activity of gut microbiome plays a role for human health. 

The PhD degree was completed at the Department of Food Science, Science and Technology, Aarhus University.

This résumé was prepared by the PhD student.

Time: Tuesday 25 April 2017 at 15.30
Place:
The canteen, Department of Food Science, Kirstinebjergvej 10, 5792 Årslev, Aarhus University
Title of dissertation:
Impact of polydextrose intake on the gut metabolome as studied by NMR fecal metabolomics
Contact information:
Santosh Lamichhane, e-mail: santosh.lamichhane@food.au.dk, tel.: +45 71573191
Members of the assessment committee:

Professor Elaine Holmes, Faculty of Medicine, Department of Surgery & Cancer, Imperial College London
Professor Søren Balling Engelsen, Department of Food Science, University of Copenhagen
Senior Researcher Marianne G. Bertelsen (chairman), Department of Food Science, Aarhus University
Main supervisor:
Associate Professor Jette F. Young, Department of Food Science, Aarhus University
Co-supervisor:
Professor Hanne Christine Bertram, Department of Food Science, Aarhus University
Assistant Professor Christian Yde, Department of Food Science, Aarhus University
Language:
The PhD dissertation will be defended in English

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1520, rooms 128-134, 8000 Aarhus C.

PhD defence
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Revised 22.06.2017