DNA nanotechnology: addressing the limitations of a promising material

PhD defence, Thursday, 6 october 2016. Mattia De Stefano

2016.10.06 | Steffi Hjerrild Iversen

Mattia De Stefano

During his PhD studies, Mattia De Stefano has worked with DNA as building material and has explored the versatility of this biopolymer in nanotechnology by creating functionalized nanostructures for nanoelectronic, biosensing and cargo-delivery applications. However, the main focus of his research has been overcoming the precarious stability that affects complex DNA architectures and limits their applicability in a wide range of contexts, especially biological environments. Specifically, he developed a new method of strands crosslinking/ligation that results in increased thermostability of DNA assemblies and improved resistance toward purification processes. The chemistry behind the method, based on disulfide bonds, is compatible with biological systems and allows obtaining highly-stable structures that can be reversed to the native state in specific conditions.

Over the past few decades, DNA has proved to be a valuable material with a wide potential applicability in nanotechnology, and this work constitutes a step toward the exploiting of its full potential as building element.

The PhD degree was completed at the Interdisciplinary Nanoscience Center (iNANO), Science and Technology, Aarhus University.

This résumé was prepared by the PhD student.

Time: Thursday, 6 October 2016 at 14.15
Place: Building 1531, room 219, Lecture Theatre D4, Department of Mathematics, Ny Munkegade 118, Aarhus University, 8000 Aarhus C.
Title of dissertation: Functionalizing DNA architectures for enhancing their application potential.
Contact information: Mattia De Stefano, e-mail: mdestef@inano.au.dk, tel.: +45 5020 3950
Members of the assessment committee:
Directeur de Recherche Professor Jean-Louis Mergny, Institut Européen de Chimie et Biologie, Pessac, France
Associate Professor Birte Vester, Department of Biochemistry and Molecular Biology, University of Southern Denmark Professor Troels Skrydstrup (chair), iNANO and Department of Chemistry, Aarhus University
Main supervisor:
Professor Kurt V. Gothelf, iNANO and Department of Chemistry, Aarhus University
The PhD dissertation will be defended in English

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1520, rooms 128-134, 8000 Aarhus C.

PhD defence
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Revised 23.03.2017