Metabarcoding improves the efficiency of monitoring soil mesofauna

PhD defence, Thursday May 11 2017. Jiayi Qin

2017.05.11 | Steffi Hjerrild Iversen

Jiayi Qin

During her study, Jiayi has applied metabarcoding in field studies of soil mesofauna and examined wood ash effects in a spruce forest. Metabarcoding is based on extraction of the pieces of faunal DNA remains in the soil, so-called eDNA, and recovers a complete species list of those soil living organisms. When employing metabarcoding Jiayi Qin showed that wood ash application up to 6 t ha-1 did not affect enchytraeid species richness. Enchytraeids are a type of small white earthworms abundant in the forest floor litter and humus layer. From laboratory experiments she learned that short-term chronic effects of wood ash differed among different soil types, and osmotic stress was the likely cause of effects while high pH and heavy metals was of minor importance.

The PhD research has applied metabarcoding as new tool for monitoring of soil biodiversity with improved efficiency, in a reliable and reproducible way.

The PhD degree was completed at the Department of Bioscience, Aarhus University.

This summary was prepared by the PhD student.

Time: Thursday 11 May 2017 at 13.00
Place: Room D2.04, Department of Bioscience, Aarhus University, Vejlsøvej 25, 8600 Silkeborg.
Title of dissertation: Effects of wood ash on soil mesofauna and application of metabarcoding
Contact information: Jiayi Qin, e-mail:, tel.: +45 50131152
Members of the assessment committee:

Associate Professor Rasmus Kjøller, Terrestrial Ecology, Department of Biology, University of Copenhagen
Professor Alfried P. Vogler, Department of Life Sciences, Imperial College London, and Natural History Museum
Senior Researcher Annette Baattrup-Pedersen (chair), Department of Bioscience, Aarhus University.
Main supervisor:
Senior Scientist Paul Henning Krogh, Department of Bioscience, Aarhus University
Professor Lars Hestbjerg Hansen, Department of Environmental Science, Aarhus University
 The PhD dissertation will be defended in English

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1520, rooms 128-134, 8000 Aarhus C.

PhD defence
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Revised 23.02.2018