RNA Origami - Building nanodevices for in vivo application

PhD defence, Tuesday 16 May 2017. Steffen Lynge Sparvath.

2017.05.16 | Steffi Hjerrild Iversen

Steffen Lynge Sparvath

Illustration of a rationally designed 3D RNA nanostructure during cotranscriptional folding: The domains of the single-stranded RNA origami nanostructure (rainbow colors) fold sequentially as the newly synthesized RNA emerges from the transcription bubble inside the T7 RNA polymerase (transparent grey surface). In the illustration the T7 RNA polymerase moves left along the DNA (light blue) and the 3’-end of the RNA base pairs with the template strand in the transcription bubble.

During his PhD studies, Steffen Sparvath researched the potential applications of RNA origami nanostructures, as nanodrugs or biosensors. RNA origami allows the creation of rationally designed RNA nanostructures that can be used to develop structural scaffolds and dynamic devices for both in vitro and in vivo applications. Steffen Sparvath studied different RNA design techniques, and his dissertation describes state-of-the-art advancements in structural RNA nanotechnology and development of functional RNA-based devices.
The new research findings contribute to the understanding of how RNA can be used as a building material to create complex RNA-based nanodevices for in vivo applications.

The PhD degree was completed at the Interdisciplinary Nanoscience Center (iNANO), Science and Technology, Aarhus University.
This résumé was prepared by the PhD student.

Time: Tuesday, 16 May 2017 at 14.15
Place: Building 1593, room 012, the Lecture Theatre, iNANO House, Gustav Wieds Vej 14, Aarhus University, 8000 Aarhus C.
Title of dissertation: RNA Origami - Developing Cotranscriptionally Folded RNA Nanostructures for in vivo Applications
Contact information: Steffen Sparvath, e-mail: sparvath@inano.au.dk, tel.: +45 2168 8818
Members of the assessment committee:
Associate Professor Arkadiusz Chworos, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Department of Bioorganic Chemistry, Lodz, Poland
Associate Professor Jeppe Vinther, Department of Biology, University of Copenhagen
Senior Researcher Morten Foss (chair), iNANO, Aarhus University
Main supervisor:
Associate Professor Ebbe Sloth Andersen, iNANO and Department of Molecular Biology and Genetics, Aarhus University
Co-supervisor:
Professor Jørgen Kjems, iNANO and Department of Molecular Biology and Genetics, Aarhus University
Language: The PhD dissertation will be defended in English

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1520, rooms 128-134, 8000 Aarhus C.

PhD defence
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Revised 19.05.2017